Article No
303-X1719M
Mouse anti Nitrotyrosine
Mouse anti Nitrotyrosine
Antibodies Primary
Specifications
| Application | WB |
| Article No | 303-X1719M |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | 2A12 |
| Clone Type | monoclonal |
| Concentration | See vial for concentration |
| Conjugation | Unconjugated |
| Description | Mouse anti Nitrotyrosine |
| Supplier | Exalpha Biologicals |
| Immunogen | 3-Nitrotyrosine-KLH |
| Isotype | IgG1 |
| Notes | Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A (SP-A) to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis. |
| Product Type | Antibodies Primary |
| Protocol | Antibody can be used for Western blotting (Suggested dilution - 1:1000) and immunohistochemistry. Optimal concentration should be evaluated by serial dilutions. |
| Research area | Cell Signaling |
| Shipping Information | Ship at ambient temperature, freeze upon arrival |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | multi species |
| Stability | See expiration date on vial |
| Storage | Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles |
| Substrate / Buffer | 100 µg monoclonal antibody in 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, 3mM sodium azide, pH 7.5 |
| Technical Specifications | Antibody can be used for Western blotting (Suggested dilution - 1:1000) and immunohistochemistry. Optimal concentration should be evaluated by serial dilutions. |
| Product Page Updated | 2023-12-29T14:25:47.027Z |
