Antibodies Primary

GFP

NEW
Mouse anti Green Fluorescent Protein (GFP)

Article No

303-X1574M

Application

WB

Size

100 µg

Clone

F56-BA1.2.3

Source / Host

mouse

Shipping Information

Ship at ambient temperature, freeze upon arrival

Product insert

Suggested protocols

Specifications

Application WB
Article No 303-X1574M
Country Availability SE, FI, DK, NO, EE, LV, LT
Clone F56-BA1.2.3
Clone Type monoclonal
Concentration See vial for concentration
Conjugation Unconjugated
Control Tissue Purified Green Fluorescent Protein
Description Mouse anti Green Fluorescent Protein (GFP)
Supplier Exalpha Biologicals
Immunogen Hybridoma produced by the fusion of splenocytes of mice immunized with full-length green fluorescent protein and mouse myeloma cells.
Isotype IgG
Notes Green Fluorescent Protein (GFP) from Jellyfish (Aequorea Victoria) is a 27kDa protein that provides strong visible fluorescence. GFP can be used as a reporter of gene expression, a tracer of cell lineage, and a fusion tag to monitor protein localization from living cells. It can also be used to access protein-protein interactions in yeast two hybrid systems and measure distances between proteins in fluorescence energy transfer (FRET).
Product Type Antibodies Primary
Purification Protein A/G Chromatography
References 1. Prasher,D.C et.al. 'Primary structure of the Aequorea victoria green-fluorescentprotein.' Gene 111 (2), 229-233 (1992) 2. Inouye,S. and Tsuji,F.I. 'Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein' FEBS Lett. 341 (2-3), 277-280 (1994) 3. Cody,C.Wet.al. 'Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein' Biochemistry 32 (5), 1212-1218 (1993
Research Area Molecular Biology
Shipping Information Ship at ambient temperature, freeze upon arrival
Size 100 µg
Source / Host mouse
Storage Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Substrate / Buffer Provided as solution in phosphate buffered saline with 0.08% sodium azide
Technical Specifications Antibody can be used for Western blotting (1-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Product Page Updated 2019-01-09T10:09:28.847Z

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