Article No
303-X1567M
Mouse anti Human Estrogen Receptor
Mouse anti Human Estrogen Receptor
Antibodies Primary
Specifications
| Application | WB, IP, GSA |
| Article No | 303-X1567M |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | TE111.5D11 |
| Clone Type | monoclonal |
| Concentration | See vial for concentration |
| Conjugation | Unconjugated |
| Control Tissue | T47D Cells |
| Description | Mouse anti Human Estrogen Receptor |
| Supplier | Exalpha Biologicals |
| Immunogen | Hybridoma produced by the fusion of splenocytes from mice immunized with the 35 kDa C-terminal fragment (aa 302-595) of human ER expressed in E. coli and mouse myeloma cells. |
| Isotype | IgG1 |
| Notes | The estrogen receptor (ER) gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. |
| Product Type | Antibodies Primary |
| Protocol | This antibody can be used gel supershift assays (neat concentration), immunoprecipitation on native and denatured lysate (2 µg/mg of protein lysate), Western blotting (1-2 µg/ml) and chromatin precipitation. Can also be used to block binding of estrogen to the estrogen receptor. Optimal concentration should be evaluated by serial dilutions. |
| Purification | Protein A/G Chromatography |
| Research area | Cell Signaling |
| Shipping Information | Ship at ambient temperature, freeze upon arrival |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | human, mouse, rat, sheep/ovine, rabbit, hamster, cow/bovine, pig/porcine, dog/canine, chicken |
| Stability | See expiration date on vial |
| Storage | Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles |
| Substrate / Buffer | Provided as solution in phosphate buffered saline with 0.08% sodium azide |
| Technical Specifications | This antibody can be used gel supershift assays (neat concentration), immunoprecipitation on native and denatured lysate (2 µg/mg of protein lysate), Western blotting (1-2 µg/ml) and chromatin precipitation. Can also be used to block binding of estrogen to the estrogen receptor. Optimal concentration should be evaluated by serial dilutions. |
| UniProt Number | P11474 |
| Product Page Updated | 2023-12-29T14:25:47.027Z |
