Antibodies Primary

Prostaglandin-E2 receptor EP2

NEW
Mouse anti Human Prostaglandin-E2 receptor EP2

Article No

303-X1491M

Application

WB

Species Reactivity

rat
cow/bovine
human

Size

100 µg

Clone

3E6

Source / Host

mouse

Product insert

Suggested protocols

Specifications

Application WB
Article No 303-X1491M
Clone 3E6
Clone Type monoclonal
Concentration See vial for concentration
Conjugation Unconjugated
Control Tissue Porcine brain lysate
Description Mouse anti Human Prostaglandin-E2 receptor EP2
Supplier Exalpha Biologicals
Immunogen Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human EP2 receptor protein and mouse myeloma cells.
Isotype IgG1
Notes Prostaglandins (PG's) are produced by the metabolism of arachidonic acid. PGE-2 is one of the five physiologically significant prostanoids known. ItÕs wide spectrum of physiologic and pharmacologic effects in various tissues are mediated through binding to the PGE-2 receptors (EP1, EP2, EP3 & EP4). These include effects on the immune, endocrine, cardiovascular, renal and reproductive systems as well as smooth muscle. It is also one of the most abundant of the prostanoid family in the brain where it plays an important role in many neural functions, particularly in newborn babies, and as a mediator of inflammation. _x000B__x000B_PGE-2 signals through a family of G-protein coupled receptors known as EP receptors. There are 4 subtypes of EP receptors, known as EP1, EP2, EP3 and EP4. EP2 receptors are 358 amino acid proteins with a short third intracellular loop. EP2 receptors stimulate adenylyl cyclase by their coupling to Gs and do not undergo PGE-2-induced internalization. The EP2 receptors is involved with the contration and relaxation of smooth muscle tissue. These receptors are mainly localized in lung and placental tissues and in smooth muscle.
Alias Names Prostaglandin E2 Receptor Subtype EP2; Provided under license from Allergan, Inc. United States Patent No. 5,716,835
Product Type Antibodies Primary
Purification Protein A/G Chromatography
References 1. Bhattacharya, M., et al. Nuclear prostaglandin receptors. Gene Ther. Mol. Biol. 1999, 4, 323-338 2. Desai, S., et al. Comparison of agonist-induced internalization of the human EP2 and EP4 prostaglandin receptors: role of the carboxyl terminus in EP4 receptor sequestration. Mol. Pharmacol. 2000, 58, 1279-1286 3. Morath, R., et al. Immunolocalization of the four prostaglandin E2 receptor proteins EP1, EP2, EP3 and EP4 in human kidney. J. Am. Soc. Nephrol. 1999, 10, 1851-1860 4. Fedyk, E.R., et al. Prostaglandin E2 receptors of the EP2 and EP4 subtypes regulate activation and differentiation of mouse B lymphocytes to IgE-secreting cells. Proc. Natl. Acad. Sci. USA 1996, 93, 10978-10983 5. Najarian, T., et al. Preservation of neural function in the perinate by high PGE(2) levels acting via EP(2) receptors. J. Appl. Physiol. 2000, 89, 777-784.
Research Area Inflammation
Size 100 µg
Source / Host mouse
Species Reactivity rat, cow/bovine, human
Storage Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Substrate / Buffer Provided as solution in phosphate buffered saline with 0.08% sodium azide
Technical Specifications This antibody can be used for Western blot analysis (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Product Page Updated 2019-01-09T10:09:28.847Z

Shipping info

The delivery time for this item is approximately 5-8 business days. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.