Antibodies Primary

Luciferase

NEW
Mouse anti Luciferase (Firefly) Protein Luciferase (N-Terminus)

Article No

303-X1171M

Application

WB, IHC

Species Reactivity

insect

Size

100 µg

Clone

Luci17

Source / Host

mouse

Shipping Information

Ship at ambient temperature, freeze upon arrival

Product insert

Suggested protocols

Specifications

Application WB, IHC
Article No 303-X1171M
Country Availability SE, FI, DK, NO, EE, LV, LT
Clone Luci17
Clone Type monoclonal
Concentration See vial for concentration
Conjugation Unconjugated
Control Tissue Purified luciferase protein
Description Mouse anti Luciferase (Firefly) Protein Luciferase (N-Terminus)
Supplier Exalpha Biologicals
Immunogen Hybridoma produced by the fusion of splenocytes from mice immunized with luciferase protein isolated from Photinus pyralis.
Isotype IgG1
Notes Analysis of gene expression is most commonly assayed by transient transfection. Systems are generally based on the use of fusion genes which are inserted into cells, and the gene expression is assayed within 48 hours after introduction of DNA. Usually the fusion consists of the promoter binding site or enhancer sequence under study which is attached to a reporter gene. The amount of the reporter protein synthesized under the experimental conditions, is presumed to reflect the ability of the sequences studied to direct or promote transcription. Several enzymes are commonly used as reporter proteins, among them are chloramphenicol acetyl transferase (CAT), -galactosidase, human growth hormone (hGH) and luciferase. Luciferase has become one of the widely used reporter enzymes. The enzyme catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg+2 and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. The use of an antibody to detect luciferase can provide an alternative detection assay which directly detects luciferase protein levels, and thus has the advantage that it does not require luciferase activity and is not dependent on rapid kinetics. Moreover, antibodies can detect the luciferase enzyme expression in situ, providing a means to study the localized signal sequences using luciferase as a reporter gene. Reacts with Luciferase (Firefly) Protein.
Product Type Antibodies Primary
Purification Protein A/G Chromatography
References 1. Aoki, Y., et al. Selective stimulation of G-CSF gene expression in macrophages by a stimulatory monoclonal antibody as detected by a luciferase reporter gene assay. J. Leukoc. Biol. 2000, 68, 757-764 2. Nicolas, M.T., et al. Immunogold labeling of luciferase in the luminous bacterium Vibrio Harveyi after fast-freeze fixation and different freeze-substitution and embedding procedures. J. Histochem. Cytochem. 1989, 37, 663-674
Research Area Molecular Biology
Shipping Information Ship at ambient temperature, freeze upon arrival
Size 100 µg
Source / Host mouse
Species Reactivity insect
Storage Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Substrate / Buffer Provided as solution in phosphate buffered saline with 0.08% sodium azide
Technical Specifications Detects luciferase protein by Western blot in C. elegans and Drosophilia melanogaster tissues, human fibroblast, mouse macrophage, kidney, liver and cortex as well as NIH3T3, Jurkat and BHR21 cell lines. Detects luciferase with little to no background signal. Optimal concentration should be evaluated by serial dilutions.
Product Page Updated 2019-01-09T10:09:28.847Z

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