Antibodies Primary

HA agarose

NEW
Mouse anti HA Tag, conjugated with agarose

Article No

303-X1029

Application

IP

Size

1 ml

Clone

12CA5

Source / Host

mouse

Shipping Information

Room Temperature

Product insert

Suggested protocols

Specifications

Application IP
Article No 303-X1029
Country Availability SE, FI, DK, NO, EE, LV, LT
Clone 12CA5
Clone Type monoclonal
Concentration See vial for concentration
Conjugation Agarose
Control Tissue HA taged proteins
Description Mouse anti HA Tag, conjugated with agarose
Supplier Exalpha Biologicals
Immunogen Balb/C mice were immunized with a 9 amino acid petide to influenza virus hemagglutinin
Isotype IgG2b
Notes A nine amino acid sequence derived from the viral hemagglutinin is frequently incorperated into recombinant proteins as an expression tag. Clone 12CA5 anti-HA is useful for western blotting and immunoprecipitation of these proteins. Shown is a typical western blot (10% SDS-PAGE) of HA-tagged Rit, a Ras-family GTPase, expressed in HEK293 cells and immunoprecipitated with anti-HA agarose (lane 1), lysate of HA-tagged Rit expressed in HEK293 cells (lane 2), and control HEK293 cells (lane 3) probed with clone 14G7 anti-Rit. Reacts with HA tagged proteins.
Alias Names Mouse anti HA, Agarose Conjugate, hemaglutinin
Product Type Antibodies Primary
Research Area Molecular Biology
Shipping Information Room Temperature
Size 1 ml
Source / Host mouse
Storage Product should be stored at 4-8ºC. DO NOT FREEZE
Substrate / Buffer Provided as 2x slurry (0.5 ml of settled gel) containing 20 mM sodium phosphate, 150 mM sodium chloride, pH 7.5, and 3 mM sodium azide
Technical Specifications Use 25µl of slurry/100µg of lysate for immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.
Product Page Updated 2019-01-09T10:09:28.847Z

Shipping info

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