Article No
303-X1029
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Application | IP |
Article No | 303-X1029 |
Country Availability | SE, FI, DK, NO, EE, LV, LT |
Clone | 12CA5 |
Clone Type | monoclonal |
Concentration | See vial for concentration |
Conjugation | Agarose |
Control Tissue | HA taged proteins |
Description | Mouse anti HA Tag, conjugated with agarose |
Supplier | Exalpha Biologicals |
Immunogen | Balb/C mice were immunized with a 9 amino acid petide to influenza virus hemagglutinin |
Isotype | IgG2b |
Notes | A nine amino acid sequence derived from the viral hemagglutinin is frequently incorperated into recombinant proteins as an expression tag. Clone 12CA5 anti-HA is useful for western blotting and immunoprecipitation of these proteins. Shown is a typical western blot (10% SDS-PAGE) of HA-tagged Rit, a Ras-family GTPase, expressed in HEK293 cells and immunoprecipitated with anti-HA agarose (lane 1), lysate of HA-tagged Rit expressed in HEK293 cells (lane 2), and control HEK293 cells (lane 3) probed with clone 14G7 anti-Rit. Reacts with HA tagged proteins. |
Alias Names | Mouse anti HA, Agarose Conjugate, hemaglutinin |
Product Type | Antibodies Primary |
Research Area | Molecular Biology |
Shipping Information | Room Temperature |
Size | 1 ml |
Source / Host | mouse |
Storage | Product should be stored at 4-8ºC. DO NOT FREEZE |
Substrate / Buffer | Provided as 2x slurry (0.5 ml of settled gel) containing 20 mM sodium phosphate, 150 mM sodium chloride, pH 7.5, and 3 mM sodium azide |
Technical Specifications | Use 25µl of slurry/100µg of lysate for immunoprecipitation. Optimal concentration should be evaluated by serial dilutions. |
Product Page Updated | 2019-01-09T10:09:28.847Z |
The delivery time for this item is approximately 5-8 business days.
If other deviations occur, this will be noted on the order confirmation or communicated via email.
Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.