Article No
303-X1029
Antibodies Primary
HA agarose
Mouse anti HA Tag, conjugated with agarose
Product insert
Suggested protocols
Specifications
| Application | IP |
| Article No | 303-X1029 |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | 12CA5 |
| Clone Type | monoclonal |
| Concentration | See vial for concentration |
| Conjugation | Agarose |
| Control Tissue | HA taged proteins |
| Description | Mouse anti HA Tag, conjugated with agarose |
| Supplier | Exalpha Biologicals |
| Immunogen | Balb/C mice were immunized with a 9 amino acid petide to influenza virus hemagglutinin |
| Isotype | IgG2b |
| Notes | A nine amino acid sequence derived from the viral hemagglutinin is frequently incorperated into recombinant proteins as an expression tag. Clone 12CA5 anti-HA is useful for western blotting and immunoprecipitation of these proteins. Shown is a typical western blot (10% SDS-PAGE) of HA-tagged Rit, a Ras-family GTPase, expressed in HEK293 cells and immunoprecipitated with anti-HA agarose (lane 1), lysate of HA-tagged Rit expressed in HEK293 cells (lane 2), and control HEK293 cells (lane 3) probed with clone 14G7 anti-Rit. Reacts with HA tagged proteins. |
| Alias Names | Mouse anti HA, Agarose Conjugate, hemaglutinin |
| Product Type | Antibodies Primary |
| Research Area | Molecular Biology |
| Shipping Information | Room Temperature |
| Size | 1 ml |
| Source / Host | mouse |
| Storage | Product should be stored at 4-8ºC. DO NOT FREEZE |
| Substrate / Buffer | Provided as 2x slurry (0.5 ml of settled gel) containing 20 mM sodium phosphate, 150 mM sodium chloride, pH 7.5, and 3 mM sodium azide |
| Technical Specifications | Use 25µl of slurry/100µg of lysate for immunoprecipitation. Optimal concentration should be evaluated by serial dilutions. |
| Product Page Updated | 2020-01-07T12:59:09.171Z |
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