Article No
303-X1017S
Application | WB, IP, ELISA, IF |
Article No | 303-X1017S |
Country Availability | SE, FI, DK, NO, EE, LV, LT |
Clone | PY20 |
Clone Type | monoclonal |
Concentration | See vial for concentration |
Conjugation | FITC |
Description | Mouse anti Phosphotyrosine, conjugated with FITC |
Supplier | Exalpha Biologicals |
Immunogen | Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein. |
Isotype | IgG2b |
Notes | The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest. |
Alias Names | Mouse anti phosphotyrosine, PY20, FITC conjugate |
Product Type | Antibodies Primary |
Protocol | A dilution of 5-10 ug/ml is suggested for immunohistochemistry or immunofluorescence. Optimal concentration should be evaluated by serial dilutions. |
Purification | Prot A/G |
Research area | Cell Signaling |
Shipping Information | Ship at ambient temperature |
Size | 50 µg |
Source / Host | mouse |
Species Reactivity | multi species |
Stability | See expiration date on vial |
Storage | Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles |
Substrate / Buffer | Provided as sterile solution in 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, 3 mM sodium azide |
Technical Specifications | A dilution of 5-10 ug/ml is suggested for immunohistochemistry or immunofluorescence. Optimal concentration should be evaluated by serial dilutions. |
Product Page Updated | 2023-12-29T14:25:47.027Z |