Article No
303-MUB2054P
Mouse anti Estrogen Receptor alpha/ERα/ERS1
Mouse anti Estrogen Receptor alpha/ERα/ERS1
Antibodies Primary
Specifications
| Application | IP |
| Article No | 303-MUB2054P |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | AER304 |
| Clone Type | monoclonal |
| Concentration | See vial for concentration |
| Conjugation | Unconjugated |
| Description | Mouse anti Estrogen Receptor alpha/ERα/ERS1 |
| Supplier | Exalpha Biologicals |
| Immunogen | AER304 is a mouse monoclonal IgG1 antibody derived by fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from a BALB/c mouse, immunized with estrogen receptor protein purified from calf uterus. |
| Isotype | IgG1 |
| Notes | Estrogen receptor (ER) is a hormone binding molecule, which belongs to the nuclear hormone family of intracellular receptors. Estrogen receptors exist as alpha and beta forms encoded by separate genes ESR1 and ESR2. ERα and ERβ share a high degree of sequence homology and are activated by the hormone 17β-estradiol and related compounds, including phytoestrogens and xenoestrogens. Both ERα and ERβ function as transcription factors which mediate many of the biological effects of estradiol at the level of gene regulation. Both receptors and are involved in the normal development and function of numerous tissues. Studies also suggest that ER’s may be involved in a range of pathological conditions including cancers, neurodegenerative diseases and cardiovascular disease. |
| Product Type | Antibodies Primary |
| Protocol | The AER304 antibody is suitable for the detection of estrogen receptor alpha by western blotting, immunoprecipitation and immunocytochemistry. In western blotting, the antibody recognises a doublet of approximately 67 kDa in non-reducing conditions. Optimal antibody dilutions for the different applications should be determined by titration. |
| Purification | Protein A/G Chromatography |
| Research area | Cell Death, Cell Proliferation |
| Shipping Information | Ship at ambient temperature. |
| Size | 0.5ml |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
| Substrate / Buffer | Each vial contains 500 µl 0.2 mg/ml purified monoclonal antibody in phosphated buffered saline (PBS) containing 50% glycerol + 0.2% bovine serum albumin (BSA) + 0.02% sodium azide. |
| Technical Specifications | The AER304 antibody is suitable for the detection of estrogen receptor alpha by western blotting, immunoprecipitation and immunocytochemistry. In western blotting, the antibody recognises a doublet of approximately 67 kDa in non-reducing conditions. Optimal antibody dilutions for the different applications should be determined by titration. |
| UniProt Number | UniProt: P03372 (Human) |
| Product Page Updated | 2023-12-29T14:25:47.027Z |
