Antibodies Primary

Neurofilament 200 kD

NEW
Mouse anti Neurofilament 200 kD

Article No

303-MUB1306P

Application

WB

Species Reactivity

cow/bovine
dog/canine
chicken
guinea pig
human
monkey
mouse
rabbit
rat
sheep/ovine
frog

Size

0.1 mg

Clone

RNF404

Source / Host

mouse

Suggested protocols

Specifications

Application WB
Article No 303-MUB1306P
Country Availability SE, FI, DK, NO, EE, LV, LT
Clone RNF404
Clone Type monoclonal
Description Mouse anti Neurofilament 200 kD
Supplier Exalpha Biologicals
Immunogen RNF404 is a Mouse monoclonal IgG2a antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a neurofilament preparation of calf brain tissue.
Isotype IgG2a
Notes Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the Mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.
Product Type Antibodies Primary
Research Area Neuroscience, Stem Cell
Size 0.1 mg
Source / Host mouse
Species Reactivity cow/bovine, dog/canine, chicken, guinea pig, human, monkey, mouse, rabbit, rat, sheep/ovine, frog
Storage The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Substrate / Buffer Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Technical Specifications RNF404 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:50 – 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:500 for immunoblotting applications.
Product Page Updated 2019-01-09T10:09:28.847Z

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