Article No
303-MUB0337P
Mouse anti Collagen Type IV
Mouse anti Collagen Type IV
Antibodies Primary
Specifications
| Application | ICC, IHC, WB |
| Article No | 303-MUB0337P |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | 1043 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | Mouse anti Collagen Type IV |
| Supplier | Exalpha Biologicals |
| Immunogen | 1043 is a Mouse monoclonal IgG1 antibody derived by fusion of Sp 2/0 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with purified Human type IV collagen. The immunogen has been isolated from Human placenta. |
| Isotype | IgG1 |
| Notes | The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasma. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antiserum is important in the differential diagnosis of bullous lesions in dermatopathology. |
| Product Type | Antibodies Primary |
| Protocol | 1043 is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 . For immunohistochemistry on paraffin sections the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. Optimal antibody dilution should be determined by titration; 1:50 – 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications. |
| Research area | Stem Cell |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
| Substrate / Buffer | Each vial contains 100 ul 1mg/ml of purified monoclonal antibody in PBS containing 0.09% sodium azide |
| Technical Specifications | 1043 is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 . For immunohistochemistry on paraffin sections the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. Optimal antibody dilution should be determined by titration; 1:50 – 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications. |
| UniProt Number | P08572, Q01955, P29400, P53420, P02462 |
| Product Page Updated | 2023-12-29T14:25:47.027Z |
