Article No
303-MAHu/IgG4/MAb(1mg)
Mouse anti Human IgG4 (pFc subclass specific)
Mouse anti Human IgG4 (pFc subclass specific)
Antibodies Secondary
Specifications
| Article No | 303-MAHu/IgG4/MAb(1mg) |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | NI 315 (HP 6206) |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | Mouse anti Human IgG4 (pFc subclass specific) |
| Supplier | Exalpha Biologicals |
| Isotype | IgG1 |
| Notes | The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc portion of the IgG4 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG4, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.` |
| Previous Article No | 303-MAHu/IgG4/MAb (1 mg) |
| Product Type | Antibodies Secondary |
| Protocol | Immunohistochemistry, immunocytochemistry, ELISA |
| Shipping Information | Ship at ambient temperature. |
| Size | 1 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Lyophilized – can be stored at +4oC. After reconstitution aliquots can be stored at -20C. The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4ºC, not refrozen, and preferably used the same day. |
| Substrate / Buffer | Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/ml. No foreign proteins added. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. |
| UniProt Number | P01833 |
| Product Page Updated | 2023-12-29T14:25:47.027Z |