Article No
303-MAHu/IgG3/TRITC
Mouse anti Human IgG3 (F(ab'')2 subclass specific), conjugated with TRITC
Mouse anti Human IgG3 (F(ab'')2 subclass specific), conjugated with TRITC
Antibodies Secondary
Specifications
| Article No | 303-MAHu/IgG3/TRITC |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | NI 330 (HP 6050) |
| Clone Type | monoclonal |
| Conjugation | TRITC |
| Description | Mouse anti Human IgG3 (F(ab'')2 subclass specific), conjugated with TRITC |
| Supplier | Exalpha Biologicals |
| Isotype | IgG1 |
| Notes | The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab’)2 part of the IgG3 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50; in ELISA from 1:200 upwards; in Western blotting from 1:400 upwards. |
| Product Type | Antibodies Secondary |
| Protocol | ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. |
| Size | 0.2 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day. |
| Substrate / Buffer | Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
| Technical Specifications | ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. |
| Product Page Updated | 2023-12-29T14:25:47.027Z |