Article No
303-MAHu/IgA2(m)2
Article No | 303-MAHu/IgA2(m)2 |
Country Availability | SE, FI, DK, NO, EE, LV, LT |
Clone | NI 194-3 (A89-040) |
Clone Type | monoclonal |
Conjugation | Unconjugated |
Description | Mouse anti Human IgA2 (A2(m)2 allotype specific) |
Supplier | Exalpha Biologicals |
Isotype | IgG1 |
Notes | The reactivity of the antiserum is restricted to the IgA2(m)2 molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA2(m)2, haemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:200 upwards; in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only. |
Product Type | Antibodies Secondary |
Protocol | Indirect immunofluorescence,ELISA. |
Size | 0.5 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4ºC, not refrozen, and preferably used the same day. |
Substrate / Buffer | Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/ml. No foreign proteins added. |
Technical Specifications | Indirect immunofluorescence,ELISA. |
Product Page Updated | 2023-12-29T14:25:47.027Z |