Article No
303-GAB/IgG(Fc)
Goat anti Bovine IgG (Fc specific)
Goat anti Bovine IgG (Fc specific)
Antibodies Secondary
Specifications
| Article No | 303-GAB/IgG(Fc) |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | Polyclonal |
| Clone Type | polyclonal |
| Conjugation | Unconjugated |
| Description | Goat anti Bovine IgG (Fc specific) |
| Supplier | Exalpha Biologicals |
| Notes | The reactivity of the antiserum is restricted to the Fc part of the IgG molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal bovine serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No precipitation reaction is obtained with purified IgA, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in bovine serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of bovine IgG from serum or plasma. |
| Product Type | Antibodies Secondary |
| Protocol | Precipitation assays. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. |
| Size | 1 ml |
| Source / Host | goat |
| Species Reactivity | cow/bovine |
| Storage | The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. |
| Substrate / Buffer | Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. |
| Technical Specifications | Precipitation assays. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. |
| UniProt Number | F1PGM1 |
| Product Page Updated | 2023-12-29T14:25:47.027Z |