Article No
303-ACdBTSA
Chicken anti Clostridium difficile Binary Toxin, Subunit A
Chicken anti Clostridium difficile Binary Toxin, Subunit A
Antibodies Primary
Specifications
| Application | FC |
| Article No | 303-ACdBTSA |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone Type | polyclonal |
| Concentration | See vial for concentration. |
| Conjugation | Unconjugated |
| Description | Chicken anti Clostridium difficile Binary Toxin, Subunit A |
| Supplier | Exalpha Biologicals |
| Format | Liquid |
| Notes | Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent. Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained, which cannot be attributed to differences in laboratory procedures, please contact us. |
| Product Type | Antibodies Primary |
| Protocol | This antibody has been tested in direct ELISA and Western blot. A 1:4000 dilution was an effective probe for 125 ng of C. difficile Binary Toxin A Subunit. The antibody titre, measured at the midpoint, was 1:56,000 of a 2.0 mg/ml solution (~90 ng/ml) using a 2 ug coating of C. difficile Binary Toxin, A Subunit. Optimal working dilutions should be determined for your particular assay conditions. |
| Purification | Egg-yolk derived IgY Affinity Purified |
| Shipping Information | Ship at ambient temperature |
| Size | 0.1 mg |
| Source / Host | chicken |
| Storage | Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles. |
| Substrate / Buffer | Phosphate buffered saline‚ pH 7.2 with 0.075% sodium azide |
| Technical Specifications | This antibody has been tested in direct ELISA and Western blot. A 1:4000 dilution was an effective probe for 125 ng of C. difficile Binary Toxin A Subunit. The antibody titre, measured at the midpoint, was 1:56,000 of a 2.0 mg/ml solution (~90 ng/ml) using a 2 ug coating of C. difficile Binary Toxin, A Subunit. Optimal working dilutions should be determined for your particular assay conditions. |
| Product Page Updated | 2023-12-29T14:25:47.027Z |
