Article No
303-10623002
Double-stranded RNA (dsRNA) ELISA kit (K1 based)
Double-stranded RNA (dsRNA) ELISA kit (K1 based)
Kits & Panels
Specifications
| Application | WB, ELISA |
| Article No | 303-10623002 |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | K1/K2 |
| Concentration | See vial for concentration. |
| Conjugation | Biotin |
| Description | Double-stranded RNA (dsRNA) ELISA kit (K1 based) |
| Supplier | Exalpha Biologicals |
| Isotype | IgG2a k, IgM k |
| Notes | Based on the use of two double-stranded RNA (dsRNA)-specific monoclonal antibodies the dsRNA Detection Kit allows sensitive and selective detection of dsRNA molecules (larger than 30-40 bp), independent of their nucleotide composition and sequence. The detection is highly specific: dsRNA can be detected in nucleic acid extracts in the presence of 1.000-10.000-fold excess of other nucleic acids. This assay works on the sandwich-ELISA principle and uses the K1 (IgG2a) mouse monoclonal antibody to dsRNA as a catcher antibody. The monoclonal antibody K2 (IgM) is used as the detector antibody. Over the past decade our double-stranded RNA (dsRNA)antibodies have been used extensively to detect and characterise plant and animal viruses with dsRNA genomes or intermediates. In addition, the anti-dsRNA antibodies can be used as a diagnostic tool to detect pathogens, including detection in paraffin-embedded fixed tissue samples (Richardson et al. 2010). The K1 monoclonal antibody recognises dsRNA with similar affinity to our widely used J2 antibody. It can be used for the histological and cytological detection of dsRNA in cells and tissues. It has proven especially useful as an alternative to J2 to resolve cross-reactions and/or remove unwanted background, in those rare experimental setups where J2 did not provide satisfactory results. K1 can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis virus, Theiler’s murine encephalomyelitis virus or Japanese encephalitis virus. It has been for the detection of dsRNA in cultured cells and in fixed paraffin-embedded histological samples (see publications). If Poly I:C needs to be detected we highly using K1 rather than J2 because K1 has a much higher affinity for this synthetic polyribonucleotide (see Schönborn et al. 1991, Fig. 2). K1 has been used successfully in immunofluorescence microscopy, in flow cytometry (FACS) and in immunocapture methods (such as dot-blot and ELISA). |
| Alias Names | dsRNA ELISA kit |
| Product Type | Kits & Panels |
| Protocol | We recommend using the kit to detect viral dsRNAs or large natural or synthetic dsRNAs of non-viral origin in nucleic acid extracts, as well as to detect the presence of undesired dsRNA molecules in artificially synthesized (m)RNA preparations. By using serial dilutions of the Poly(I:C) dsRNA standard (included in the kit) for calibration, quantitative estimates can also be made._x000D_ For the exact detection protocol we refer to the kit manual that can be downloaded from our website,_x000D_ _x000D_ Caution: The Poly(I:C) dsRNA positive control included in this sandwich ELISA kit is not intended to be used as a quantitative standard for other dsRNA preparations. The anti dsRNA antibodies K1 and/or K2 used in this kit may exhibit a different degree of reactivity with different dsRNAs obtained from natural sources. It is therefore only intended to be used as a positive control to see if the ELISA has been executed correctly and that the test shows a linear relationship between the amount of dsRNA and the read out for the OD450. |
| Purification | Egg-yolk derived IgY Affinity Purified |
| Shipping Information | The Double-stranded RNA (dsRNA) ELISA kit components are shipped on blue ice. |
| Size | Reagents for 200 tests |
| Source / Host | mouse |
| Species Reactivity | bacteria |
| Storage | Upon receipt, store entire kit at -20ºC. Once the kit is thawed, you may keep it at 4ºC for 5 days. For long-term storage, it is recommended to aliquot and freeze the antibody and dsRNA components at –20ºC. |
| Substrate / Buffer | Affinity purified antiserum in phosphate buffered saline‚ pH 7.2‚ 50% glycerol with 0.1% 2-chloroacetamide. |
| UniProt Number | Q00496 |
| Product Page Updated | 2023-12-29T14:25:47.027Z |
