Article No
303-0027S
Mouse anti Human CD2 FITC - CD7 PE
Mouse anti Human CD2 FITC - CD7 PE
Antibodies Primary
Specifications
| Application | IHC |
| Article No | 303-0027S |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone Type | monoclonal |
| Concentration | Titered for flow cytometry |
| Conjugation | FITC, RPE |
| Description | Mouse anti Human CD2 FITC - CD7 PE |
| Supplier | Exalpha Biologicals |
| Immunogen | CD2=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with t lymphocytes activated by mixed lymphocyte culture. CD7=Derived from the hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with T-acute lymphoblastic leukemia (T-ALL) cells. |
| Notes | Identification of human T cells and subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen. Identification of human T lymphocytes in multiple stages of T cell development, including a major subset of mature peripheral T cell. CD7 antigen is often increased on T leukemic cells. The CD7 molecule is a 40,000 M.W. surface antigen that is expressed on T-Lymphoid and myeloid precursors in fetal liver and bone marrow. |
| Alias Names | CD2 FITC - CD7 PE |
| Product Type | Antibodies Primary |
| Protocol | PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB /100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. |
| Purification | Protein A/G Chromatography |
| Shipping Information | Ship at ambient temperature, do not freeze, refrigerate upon arrival |
| Size | 50 Tests |
| Source / Host | mouse |
| Species Reactivity | human |
| Stability | See expiration date on vial |
| Storage | Product should be stored at 4-8ºC. DO NOT FREEZE |
| Substrate / Buffer | Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein |
| Technical Specifications | PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB /100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. |
| UniProt Number | P06729, P09564 |
| Product Page Updated | 2023-12-29T14:25:47.027Z |
