Article No
260-6292
Goat anti-Mouse IgG Fc, HRP, AffiPure
Goat anti-Mouse IgG Fc, HRP, AffiPure
Assay & Detection
Specifications
| Application | ELISA |
| Article No | 260-6292 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone Type | polyclonal |
| Description | Goat anti-Mouse IgG Fc, HRP, AffiPure |
| Supplier | ImmunoChemistry Technologies |
| Notes | ICT’s HRP Goat Anti Mouse IgG Fc is suitable for ELISA, Western blotting, and histology techniques. Goats were immunized with a purified preparation of mouse IgG Fc fragment that was obtained from a purified normal mouse IgG antibody protein pool. The hyperimmune IgG fraction was initially pre-adsorbed over solid phase gels bearing covalently coupled mouse Fab protein. Subsequently, the goat anti-Mouse IgG Fc antibody was adsorbed to and eluted from a second solid phase immunoaffinity purification gel containing covalently coupled mouse IgG Fc protein. Affinity purified, anti-mouse IgG Fc specific goat IgG was covalently coupled with HRP using a maleimide (M)- facilitated conjugation technique. In this process, free sulfhydryl groups are added to the anti-mouse IgG Fc prep just prior to its reaction with a 4-fold molar excess of HRP-M. This process produces a Goat IgG HRP conjugate that contains between 2 and 4 HRP molecules per IgG molecule. Using ID and IEP analysis techniques, the IgG component of this Goat anti-Mouse IgG Fc HRP conjugate was specific for normal mouse IgG and was non-reactive with IgM or IgE serum proteins. The conjugate is dissolved in a Tris based conjugate stabilization buffer containing 50 ppm ProClin 300 anti-microbial agent. |
| Product Type | Assay & Detection |
| Shipping Information | BLUE ICE |
| Size | 1.0 mg |
| Source / Host | goat |
| Species Reactivity | mouse |
| Storage | 4°C |
| Technical Specifications | • Molecular Weight: 230 – 310 Kd • Recommended dilutions: Western blotting: 1:500 – 1:2,000 ELISA: 1:5,000 – 1:20,000 • Storage: 24 months from receipt when stored at 2-8°C • Shipping: Ships overnight (domestic), International Priority Shipping |
| Product Page Updated | 2024-01-03T12:33:39.983Z |
