Article No
246-MUB1319P
Mouse anti Neu-Oncogen (C-erb B2)
Mouse anti Neu-Oncogen (C-erb B2)
Antibodies Primary
Specifications
| Application | IHC, IP, WB |
| Article No | 246-MUB1319P |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | 3B5 |
| Clone Type | monoclonal |
| Description | Mouse anti Neu-Oncogen (C-erb B2) |
| Supplier | Nordic - MUbio |
| Format | Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. |
| Isotype | IgG1 |
| Notes | C-erbB-2 (erythroblastosis oncogene B), also known as HER2 (Human Epidermal Growth Factor Receptor 2), or Neu, CD340 and p185 is a protein that in Humans is encoded by the ERBB2 gene. AmplifiCation or overexpression of this gene has been shown to play an important role in the pathogenesis and progression of certain aggressive types of breast cancer, as well as many other epithelial malignancies and brain tumors. In recent years it has become an important biomarker and target of therapy for disease. ERBB2 is a known proto-oncogene loCated at the long arm of Human chromosome 17 (17q21-q22). The oncogene was found to code for EGFR. Gene cloning showed that HER2, Neu and ErbB-2 are all encoded by the same gene. The ErbB family is composed of plasma membrane-bound receptor tyrosine kinases, that contain an extracellular ligand binding domain, a transmembrane domain and an intracellular domain that can interact with a multitude of signaling molecules. HER2 can heterodimerise with any of the other three receptors and is considered to be the preferred dimerisation partner of the other ErbB receptors. Dimerisation results in the autophosphorylation of tyrosine residues within the cytoplasmic domain of the receptors and initiates a variety of signaling pathways. |
| Previous Article No | 246-MUB1319P |
| Product Type | Antibodies Primary |
| Protocol | 3B5 is useful for immunohistochemistry on frozen and paraffin-embedded tissues, immunocytochemistry, flow cytometry, immunoprecipitation and immunoblotting. Optimal antibody dilution should be determined by titration. Recommended range is 1:100 – 1:500 for immunohistochemistry and 1:250 – 1:1000 for immunoblotting applications. For appliCation on paraffin embedded tissue antigen retrieval by boiling in 10mM citRate buffer pH 6.0 is recommended. |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human, monkey, mouse, rat |
| Storage | The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
| Substrate / Buffer | Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. |
| Technical Specifications | Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. |
| UniProt Number | P04626 |
| Product Page Updated | 2024-01-31T10:35:40.347Z |
