Article No
246-MAHu/IgG3
Mouse anti Human IgG3 (F(ab'')2 subclass specific)
Mouse anti Human IgG3 (F(ab'')2 subclass specific)
Antibodies Secondary
Specifications
| Article No | 246-MAHu/IgG3 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | NI 330 (HP 6050) |
| Clone Type | monoclonal |
| Cross Reactivity | The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species. |
| Description | Mouse anti Human IgG3 (F(ab'')2 subclass specific) |
| Supplier | Nordic - MUbio |
| Format | Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration after solution is 1.0 mg/ml. No foreign proteins added. |
| Isotype | IgG1 |
| Notes | The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab’)2 portion of the IgG3 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG3, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only. |
| Previous Article No | 246-MAHu/IgG3 |
| Product Type | Antibodies Secondary |
| Protocol | Indirect immunofluorescence,ELISA. |
| Research area | Immunology |
| Size | 0.5 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4ºC, not refrozen, and preferably used the same day. |
| Substrate / Buffer | Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration after solution is 1.0 mg/ml. No foreign proteins added. |
| Technical Specifications | Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration after solution is 1.0 mg/ml. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. |
| Product Page Updated | 2024-01-31T10:35:40.347Z |
