Article No
246-MAHu/IgAc/FITC
Application | FC, IF |
Article No | 246-MAHu/IgAc/FITC |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Clone | NI 69 (A89-034) and NI 184 (A89-035) |
Clone Type | monoclonal |
Conjugation | FITC |
Cross Reactivity | The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species. |
Description | Mouse anti Human IgA1+IgA2 , conjugated with FITC |
Supplier | Nordic - MUbio |
Format | Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
Isotype | IgG1 |
Notes | The reactivity of the antiserum is restricted to determinants on the CH2 domain of IgA as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining of cytoplasmic IgA, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 to 1:100; in Western blotting from 1:200 upwards. |
Previous Article No | 246-MAHu/IgAc/FITC |
Product Type | Antibodies Secondary |
Protocol | ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. |
Find out more about our research areas Biochemical, Immunology, Molecular Biology and Pathology | Immunology |
Size | 0.2 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day. |
Substrate / Buffer | Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
Technical Specifications | Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. |
Product Page Updated | 2024-01-31T10:35:40.347Z |