Article No
246-MAHu/BJK/TRITC
Application | IF |
Article No | 246-MAHu/BJK/TRITC |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Clone | NI 285, NI 250 |
Clone Type | monoclonal |
Conjugation | TRITC |
Cross Reactivity | The antibody does not react with any other component of the Human immunoglobulin system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species. |
Description | Mouse anti Human Bence Jones kappa (surface and hidden determinants), conjugated with TRITC |
Supplier | Nordic - MUbio |
Format | Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
Isotype | IgG1 |
Notes | The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining and ELISA and immunoblotting using an indirect technique with monoclonal anti TRITC labelled with peroxidase. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only. |
Previous Article No | 246-MAHu/BJK/TRITC |
Product Type | Antibodies Secondary |
Protocol | ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. |
Research area | Immunology |
Size | 0.2 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day. |
Substrate / Buffer | Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
Technical Specifications | Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. |
Product Page Updated | 2024-01-31T10:35:40.347Z |