Article No
246-GARa/IgG(H+L)/PO
Application | WB, ELISA |
Article No | 246-GARa/IgG(H+L)/PO |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Clone | Polyclonal |
Clone Type | polyclonal |
Conjugation | HRP |
Cross Reactivity | Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion an reaction has been observed with ferret Serum. |
Description | Goat anti Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains), conjugated with Horseradish peroxidase |
Supplier | Nordic - MUbio |
Format | Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. |
Notes | The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in rat serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
Previous Article No | 246-GARa/IgG(H+L)/PO |
Product Type | Antibodies Secondary |
Protocol | ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting. |
Size | 1 ml |
Source / Host | goat |
Species Reactivity | rat |
Storage | The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immuniconjugate. |
Substrate / Buffer | Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. |
Technical Specifications | Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. |
Product Page Updated | 2024-01-31T10:35:40.347Z |