Article No
246-GARa/IgG(Fc)/TRITC
Goat anti Rat IgG1 IgG2a IgG2b IgG2c (Fc specific), conjugated with TRITC
Goat anti Rat IgG1 IgG2a IgG2b IgG2c (Fc specific), conjugated with TRITC
Antibodies Secondary
Specifications
| Application | IF |
| Article No | 246-GARa/IgG(Fc)/TRITC |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | Polyclonal |
| Clone Type | polyclonal |
| Conjugation | TRITC |
| Cross Reactivity | Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this product has not been tested in detail. |
| Description | Goat anti Rat IgG1 IgG2a IgG2b IgG2c (Fc specific), conjugated with TRITC |
| Supplier | Nordic - MUbio |
| Format | TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. |
| Notes | The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal rat plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and IgG/Fab fragments or any other serum protein. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Previous Article No | 246-GARa/IgG(Fc)/TRITC |
| Product Type | Antibodies Secondary |
| Protocol | ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. |
| Size | 1 ml |
| Source / Host | goat |
| Species Reactivity | rat |
| Storage | The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. |
| Substrate / Buffer | TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. |
| Technical Specifications | TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. |
| Product Page Updated | 2024-01-31T10:35:40.347Z |
