Article No
246-GAHu/IgG(H+L)/Bio
Goat anti Human IgG (heavy and light chains), conjugated with Biotin
Goat anti Human IgG (heavy and light chains), conjugated with Biotin
Antibodies Secondary
Specifications
| Application | ELISA, IF |
| Article No | 246-GAHu/IgG(H+L)/Bio |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | Polyclonal |
| Clone Type | polyclonal |
| Conjugation | Biotin |
| Cross Reactivity | Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. This antiSerum has been tested for cross-reactivity in double radial immunodiffusion with the following results: Bovine - Dog + Horse ± pigeon - Sheep - Cat + Guinea Pig + Monkey ++ Rabbit + Swine + Chicken - Hamster ± Mouse ± Rat + Turkey - A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques. |
| Description | Goat anti Human IgG (heavy and light chains), conjugated with Biotin |
| Supplier | Nordic - MUbio |
| Format | Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added. |
| Notes | The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of human origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Previous Article No | 246-GAHu/IgG(H+L)/Bio |
| Product Type | Antibodies Secondary |
| Protocol | ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting. |
| Research area | Immunology |
| Size | 1 ml |
| Source / Host | goat |
| Species Reactivity | human |
| Storage | The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. |
| Substrate / Buffer | Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added. |
| Technical Specifications | Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. |
| Product Page Updated | 2024-01-31T10:35:40.347Z |
