Article No
246-10090100
Double stranded RNA control Poly(I:C)
Double stranded RNA control Poly(I:C)
Lysates
Specifications
| Application | ELISA, Dot blot |
| Article No | 246-10090100 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | Double stranded RNA control Poly(I:C) |
| Supplier | Nordic - MUbio |
| Notes | The low molecular weight Poly(I:C) dsRNA sequence is intended to be used as a positive control in double-stranded (ds)RNA detection. The sequence does not contain any viral or potentially transforming elements. Positive control for dsRNA detection by anti dsRNA monoclonal J2, J5, K1 and K2 antibodies. Caution: The Poly(I:C) dsRNA positive control, to be combined with our monoclonal anti dsRNA antibodies J2, J5, K1 and K2 in both the direct AN-ELISA and sandwich ELISA, is not intended to be used as a quantitative standard for other dsRNA preparations. The anti dsRNA antibodies may exhibit a different degree of reactivity with different dsRNAs obtained from natural sources. It is therefore only intended to be used as a positive control to see if the ELISA has been executed correctly and that the test shows a linear relationship between the amount of dsRNA and the read out, for example the OD450, in the 4-parameter analysis. It cannot be used to determine the concentration of a different type of dsRNA. The only proper standard for each specific application is the purified dsRNA under investigation. |
| Alias Names | Double stranded RNA (dsRNA) control/Poly(I):Poly(C) |
| Product Type | Lysates |
| Protocol | Double stranded RNA control Poly(I:C) is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturing polyacrylamide and agarose gels. We suggest to use it as a homogenous, well-characterized positive control for different dsRNA detection methods, e.g. for ELISA and dot-blots. The optimum working dilution of the Poly(I:C) dsRNA control for any specific application should be established by titration. |
| Purity | Absorbance spectrum |
| Shipping Information | Ship on blue ice. |
| Size | 50 µg |
| Stability | Shelf Life: 24 months |
| Storage | Store at -20 °C to -80 °C upon arrival, avoid freeze/thaw cycles, store in aliquots. |
| Product Page Updated | 2024-01-31T10:35:40.347Z |
