Article No
182-HM2041-20UG
BPI, Human, mAb 3F9 – 20 µg
BPI, Human, mAb 3F9 – 20 µg
Antibodies Primary
Specifications
| Application | IA |
| Article No | 182-HM2041-20UG |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | 3F9 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | BPI, Human, mAb 3F9 – 20 µg |
| Supplier | Hycult biotechnology |
| Format | 0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide. |
| Isotype | IgG1 |
| Notes | The monoclonal antibody 3F9 reacts specifically with full length human natural and recombinant Bactericidal Permeability Increasing protein (BPI). The antimicrobial protein BPI is a 55 kDa protein found in the primary (azurophilic) granules of human neutrophils and has also been detected on surface of neutrophils, small intestinal and oral epithelial cells. BPI is a bactericidal compound that is present in polymorphonuclear cells (PMN) and in lower levels in the specific granules of eosinophils. BPI possesses high affinity toward the lipid A region of lipopolysaccharides (LPS) that comprise the outer leaflet of the gram-negative bacterial outer membrane. Binding of BPI to the lipid A moiety of LPS exerts multiple anti-infective activities against gram-negative bacteria: 1) cytotoxicity via sequential damage to bacterial outer and inner lipid membranes, 2) neutralization of gram-negative bacterial LPS, 3) opsonization of bacteria to enhance phagocytosis by neutrophils. Airway epithelial cells constitutively express the BPI gene and produce the BPI protein and, therefore, BPI may be a critical determinant in the development of LPS-triggered airways disease. Inflammation induced by LPS possibly contributes to the development of rapid airflow decline, a serious and often fatal complication of hematopoietic cell transplantation. Furthermore, a 21 kDa bioactive recombinant fragment of BPI, rBPI21, was shown to confer a survival advantage against invasive pneumococcal disease by binding to the gram-positive bacterial pathogen, pneumolysin. The monoclonal antibody 3F9 recognizes only free BPI and does not interact with BPI that has formed a complex with LPS. |
| Previous Article No | 182-HM2041-20UG, HM2041-20UG |
| Product Type | Antibodies Primary |
| Research area | Microbiology, Immunology |
| Shipping Information | Room Temperature |
| Size | 20 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year. |
| Substrate / Buffer | 0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide. |
| Product Page Updated | 2024-04-05T09:42:44.568Z |