Article No
182-HM1053F-100UG
Specifications
| Application | FC, FS, IA, IF |
| Article No | 182-HM1053F-100UG |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | 103-A1 |
| Clone Type | monoclonal |
| Conjugation | FITC |
| Description | Nectin-3 |
| Supplier | Hycult biotechnology |
| Format | 1 ml (100 μg/ml) 0.2 μm protein G purified FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide. |
| Immunogen | Extracellular domain 50-400 aa of mouse Nectin-3 fused with IgG Fc (Ref.1). |
| Isotype | IgG2a |
| Notes | The monoclonal antibody 103-A1 recognizes mouse nectin-3. Nectin-3 is a 83 kDa type I transmembrane glycoprotein. Nectin, originally isolated as poliovirus receptor-related protein (PRR), is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectins are calciumindependent immunoglobulin-like cell-cell adhesion molecules consisting of four members, nectin 1-4. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in testis, in cooperation with, or independently of, cadherins. Both nectin-2 and nectin-3 are ubiquitously expressed, whereas nectin-1 is abundantly expressed in brain. Nectin-2 and -3 are expressed in cells where cadherin is not expressed, such as blood cells and spermatids. All members of the nectin family have two or three splice variants. For nectin-3, three isoforms exist: nectin-3α, -3β and -3g of which nectin-3α is the largest. Nectin-3, also known as PRR3, is a transmembrane protein that is predominantly expressed in testis and placental tissues as well in many cell lines. Nectin interacts in vivo with both long and short isoforms of afadin, an actin binding protein, at cadherin-based cell-cell adherence junctions in various tissues and cell lines. Furthermore, the ectodomains of nectin-3 and CD155 (Poliovirus Receptor) have shown strong affinity to each other. Injection of antibody 103-A1 into lumen of seminiferous tubules leads to disruption of the actin filaments in Sertoli cells at the Sertoli-maturing spermatid ectoplasmic specialization and exfoliation of maturing spermatids form the seminiferous epithelium. |
| Alias Names | Poliovirus receptor-related protein 3, Nectin cell adhesion molecule 3, CD113 Gene name: Nectin3, Pvrl3 |
| Previous Article No | 182-HM1053F, 182-HM1053F-100UG, HM1053F-100UG |
| Product Type | Antibodies Primary |
| Research area | Immunology |
| Shipping Information | Room Temperature |
| Size | 100µg |
| Source / Host | rat |
| Species Reactivity | mouse |
| Storage | Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. |
| Substrate / Buffer | 0.2 ml (100 μg/ml) 0.2 μm protein G purified FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide. |
| Product Page Updated | 2023-12-29T14:10:53.573Z |
