Article No
177-CL8917AP
Anti-Mouse CD18 , Purified (Clone C71/16) (rat IgG2a)
Anti-Mouse CD18 , Purified (Clone C71/16) (rat IgG2a)
Antibodies Primary
Specifications
| Application | FC |
| Article No | 177-CL8917AP |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone | C71/16 |
| Clone Type | monoclonal |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-Mouse CD18 , Purified (Clone C71/16) (rat IgG2a) |
| Supplier | Cedarlane Laboratories |
| Notes | Anti-Mouse CD18 , Purified (Clone C71/16) (rat IgG2a) |
| Product Type | Antibodies Primary |
| Protocol | Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium (CL5030). 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1x106 cells, representing 1 test). 4. To each tube, add 1.0 μg* of CL8917AP . 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 μl of secondary antibody CLCC40001 (FITC Goat anti-rat IgG (H+L)) at 1:500 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 μl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls). |
| Shipping Information | BLUE ICE |
| Size | 200 ug |
| Source / Host | rat |
| Species Reactivity | mouse |
| Storage | 4°C |
| Substrate / Buffer | 200 μg purified Ig buffered in PBS and 0.02% sodium azide (NaN3) |
| Product Page Updated | 2024-01-03T12:00:43.130Z |
