Baby Rabbit Complement – Tissue Culture Grade is collected aseptically from 3-4 week old rabbits. The pooled sera is then thoroughly tested to ensure that the complement is sterile, mycoplasma-free, virus-free and is low in endotoxin. 3-4 week old rabbit complement has low heterophile activity which results in low background cytotoxicity, making this product ideal for use in cytotoxicity, depletion and opsinization assays.
Serum & Plasma
Complement Titre: Each lot is tested to ensure high activity with low inherent toxicity for complement dependent assays. Hemolytic titre is determined in a sheep RBC Hemolytic assay using presensitized sRBC. Minimum acceptable hemolytic titre is 1:16. Cytotoxic titre is determined in a lymphocytotoxicity assay using an anti-human CD3 antibody. Results are reported as the last dilution to produce greater than 50% cellular cytotoxicity. Minimum acceptable cytotoxic titre is 1:8. Endotoxin Testing: The Endotoxin level in the sample is determined using the Limulus Amebocyte Lysate test (Pyrotell gel clot method). The maximum allowed limit is ≤1.0 EU/ml. Sterility Testing: 2 week culture in Trypticase Soy Broth and Thioglycollate Media at 37ºC. General Safety Testing: This test is performed by an injection of 0.5 ml into mice and recording weights and general health for 7 days. Mycoplasma Testing: Preformed by a 30 day culture in Mycoplasma broth (containing supplement). Viral Studies: Samples are tested with atleast six cell types: monkey kidney primary (MK-1), Hep-2, human foreskin (HFS), human embryonic lung (HEL), Madin-Darby canine kidney (MDCK), and Rhabdomyosarcoma (RD).
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The delivery time for this item is approximately 5-8 business days. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.