Article No
173-24446
Application | EM, ICC, IHC, IF |
Article No | 173-24446 |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
Clone Type | polyclonal |
Conjugation | Unconjugated |
Description | 5-HTP (5-Hydroxytryptophan) Antibody |
Supplier | ImmunoStar |
Format | Whole Serum |
Immunogen | 5-HTP |
Notes | The 5-HT Antibody was raised in rabbit against 5-HTP coupled to BSA with paraformaldehyde. The antibody has a proven maximum biotin-streptavidin/HRP staining at a 1/1000 - 1/2000 dilution in rat raphe nuclei. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Shipper and Tilders, 1983). Results showed that the 5-HTP antibody dose dependently stained 5-HTP but did not stain any concentration of 5-HT or 5-HIAA. The antiserum was also tested by pre-adsorption with indole/paraformaldehyde/BSA conjugates. Staining was completely blocked by pre-adsorption with 5-HTP conjugate and unaffected by 5-HIAA or 5-HT conjugate. |
Previous Article No | 173-24446, 24446 |
Product Type | Antibodies Primary |
Research area | Metabolism |
Size | 100 µl |
Source / Host | rabbit |
Species Reactivity | rat |
Product Page Updated | 2024-01-03T12:30:51.564Z |