Article No
173-20080
5-HT (Serotonin) Rabbit Antibody
5-HT (Serotonin) Rabbit Antibody
Antibodies Primary
Specifications
| Application | IF, IHC, EM, ICC, WB |
| Article No | 173-20080 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone Type | polyclonal |
| Conjugation | Unconjugated |
| Description | 5-HT (Serotonin) Rabbit Antibody |
| Supplier | ImmunoStar |
| Format | Whole Serum |
| Immunogen | Serotonin |
| Notes | The 5-HT Rabbit Antibody was raised against serotonin coupled to BSA with paraformaldehyde. The ImmunoStar serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, raphe nuclei and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000-1/2,000 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/20,000-1/40,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 25 ug of serotonin/BSA. Cross reactivity of Serotonin antisera was examined. With 5µg, 10µg and 25µg amounts the following substances did not react with Serotonin antisera diluted 1/20,000 using the Bn-SA/HRP labeling method: 5-hydroxytryptophan, 5-hydroxyindole -3- acetic acid, and dopamine. |
| Previous Article No | 173-20080, 20080 |
| Product Type | Antibodies Primary |
| Research area | Metabolism |
| Size | 100 µl |
| Source / Host | rabbit |
| Species Reactivity | chicken, frog, guinea pig, hamster, human, monkey, mouse, rat, zebrafish, insect, cat/feline, other vertebrates, other marine species |
| Product Page Updated | 2024-01-03T12:30:51.564Z |
