Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to llama IgG1 or IgG3 are observed.
Antibodies Isotype Control
Source / Host
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Substrate / Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Llama IgG2 isotype control can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
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