Article No
155-XA-1000
Specifications
| NCBI Number | 14103 |
| Application | FC, IHC |
| Article No | 155-XA-1000 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone | A11 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | FasL Antibody [A11] |
| Supplier | ProSci |
| Entrez Gene ID | 14103 |
| Format | Liquid |
| Gene Symbol | Fasl |
| Immunogen | FasL (monoclonal A11) antibody was raised against a synthetic peptide corresponding to amino acids 196-220 of mouse FasL. |
| Isotype | IgM |
| Alias Names | Apt1lg1, Cd95l, Fasl, gld, Tnfsf6, Tumor necrosis factor ligand superfamily member 6, CD95 ligand, CD95-L, CD178, CD95L, Fas-L, Faslg, APT1LG1 |
| Previous Article No | 155-XA-1000-0.1 |
| Product Type | Antibodies Primary |
| Protocol | FasL antibody can be used for the detection of mouse FasL by flow cytometry and immunohistochemistry (frozen sections and paraffin sections).Method: Cryostat sections (6-8 µm) were air dried, fixed in 4% paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at room temperature (RT) with 20 µg/mL of A11 antibody in PBS, 0.2% BSA, 0.05% Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.Paraffin sections: Dewax in xylene (2x10 min), EtOH 100% (2x10 min), block endogeneous peroxidase (0.6% H2O2 in Methanol 10 min), rehydrate through graded EtOH (100%, 95%, 70%), rinse in H20, unmask epitopes by heating slides in citrate buffer (0.01M, pH 6.0) 2x5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 µg/mL antibody, wash in PBS, incubate in anti-rat IgM HRP, wash in PBS, develop in appropriate substrate solution (DAB or AEC). |
| Purification | FasL (monoclonal A11) antibody was purified to ≥95% in SDS-PAGE. |
| Research area | Cell Death |
| Shipping Information | BLUE ICE |
| Size | 0.1 mg |
| Source / Host | rat |
| Species Reactivity | mouse |
| Storage | FasL antibody can be stored at 4°C, stable for one year. |
| Substrate / Buffer | PBS containing 0.02% sodium azide |
| UniProt Number | P41047 |
| Product Page Updated | 2024-01-03T13:34:25.701Z |
