Article No
155-99-238
Specifications
| NCBI Number | 396249 |
| Accession Number | P31043 |
| Application | Block, CMCD, FC, IHC, IP |
| Article No | 155-99-238 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone | AV7 |
| Clone Type | monoclonal |
| Concentration | 0.5 mg/ml |
| Conjugation | Biotin |
| Description | CD28 Antibody [AV7] (Biotin) |
| Supplier | ProSci |
| Entrez Gene ID | 396249 |
| Format | Liquid |
| Gene Symbol | CD28 |
| Immunogen | Chicken peripheral blood lymphocytes |
| Isotype | IgG1 |
| Notes | Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on antigen presenting cells. Peripheral gammadelta+ T cells are CD28 negative. Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A, or monoclonal antibodies against the avian T cell receptor (TCR). Both Vbeta1- and Vbeta2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A but inhibited by herbimycin A suggesting that CD28 employs a signaling pathway at least partly distinct from that triggered by TCR/CD3. The monoclonal antibody AV7 also reacts with turkey CD28 as demonstrated by flow cytometry. |
| Previous Article No | 155-99-238-0.5 |
| Product Type | Antibodies Primary |
| Protocol | CD28 Antibody [AV7] for use in flow cytometry, immunohistochemistry / immunocytochemistry, and immunoprecipitation assays. |
| Research area | Immunology |
| Shipping Information | BLUE ICE |
| Size | 0.5 mg |
| Source / Host | mouse |
| Species Reactivity | bird/avian, chicken |
| Storage | 4°C |
| Substrate / Buffer | Supplied in PBS/NaN3 |
| Technical Specifications | CD28 Antibody [AV7] for use in flow cytometry, immunohistochemistry / immunocytochemistry, and immunoprecipitation assays. |
| UniProt Number | P31043 |
| Product Page Updated | 2024-01-03T13:34:25.701Z |
