Article No
155-40-158-0.002
PDGF-BB Recombinant Protein
PDGF-BB Recombinant Protein
Proteins & Peptides
Specifications
| NCBI Number | 5155 |
| Accession Number | NP_002599.1 |
| Article No | 155-40-158-0.002 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Description | PDGF-BB Recombinant Protein |
| Supplier | ProSci |
| Entrez Gene ID | 5155 |
| Format | Lyophilized |
| Gene Symbol | PDGFB |
| Notes | PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids). |
| Alias Names | SIS, SSV, IBGC5, PDGF2, c-sis, PDGF-2, SIS, Platelet-derived growth factor subunit B, PDGF subunit B |
| Product Type | Proteins & Peptides |
| Purity | >98% |
| Research area | Cell Proliferation |
| Sequence | SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT |
| Shipping Information | RT |
| Size | 0.002mg |
| Storage | The lyophilized PDGF-BB recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted PDGF-BB is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing PDGF-BB recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
| Technical Specifications | Biological activity was determined by the dose - dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0 - 3.0 ng/mL. |
| UniProt Number | P01127 |
| Product Page Updated | 2024-01-03T13:15:21.507Z |
