Article No
155-33-401
Specifications
| NCBI Number | 3385 |
| Application | FC, IF, IHC, WB |
| Article No | 155-33-401 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone | 101-1D2 |
| Clone Type | monoclonal |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Description | ICAM-3 Antibody [101-1D2] |
| Supplier | ProSci |
| Entrez Gene ID | 3385 |
| Format | Liquid |
| Gene Symbol | ICAM3 |
| Immunogen | Stimulated human leukocytes were used as the immunogen for the ICAM-3 antibody. |
| Isotype | IgG2a k |
| Notes | Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This mAb is excellent for staining of formalin/paraffin tissues. |
| Alias Names | Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, CD50, ICAM3 |
| Previous Article No | 155-33-401-100 |
| Product Type | Antibodies Primary |
| Protocol | Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1) Optimal dilution of the ICAM-3 antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
| Purification | Protein G Purified |
| Research area | Immunology |
| Shipping Information | BLUE ICE |
| Size | 100 ug |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C |
| Substrate / Buffer | PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
| UniProt Number | P32942 |
| Product Page Updated | 2024-01-03T13:34:25.701Z |
