Article No
155-33-149
Kappa Light Chain
Kappa Light Chain Antibody [L1C1]
Antibodies Primary
Specifications
| NCBI Number | 3514 |
| Application | FC, IF, IHC, WB |
| Article No | 155-33-149 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone | L1C1 |
| Clone Type | monoclonal |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Description | Kappa Light Chain Antibody [L1C1] |
| Supplier | ProSci |
| Entrez Gene ID | 3514 |
| Format | Liquid |
| Gene Symbol | IGKC |
| Immunogen | Human B-Lymphoma Cells were used as the immunogen for this Kappa light chain antibody. |
| Isotype | IgG1 k |
| Notes | This antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. |
| Previous Article No | 155-33-149-100 |
| Product Type | Antibodies Primary |
| Protocol | Flow Cytometry: 0.5-2.0 ug/million cells IF: 1-2 ug/ml WB: 0.5-1 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 min at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
| Purification | Protein G Purified |
| Research area | Immunology |
| Shipping Information | BLUE ICE |
| Size | 100 ug |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C |
| Substrate / Buffer | PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
| UniProt Number | P01601, P01834 |
| Product Page Updated | 2024-01-03T13:34:25.701Z |
