Antibodies Primary

AIF

NEW
AIF Antibody

Article No

155-2267-0.02

Application

ELISA, WB, IHC-P

Species Reactivity

human
mouse
rat

Size

0.02 mg

Source / Host

rabbit

Product insert

Suggested protocols

Specifications

MW Predicted: 27, 36, 67 kDa

Observed: 71 kDa
Application ELISA, WB, IHC-P
Article No 155-2267-0.02
Clone Type polyclonal
Concentration 1 mg/mL
Conjugation Unconjugated
Description AIF Antibody
Supplier ProSci incorporated
Format Liquid
Immunogen AIF antibody was raised against a 15 amino acid peptide near the center of human AIF.

The immunogen is located within amino acids 500 - 550 of AIF.
Isotype IgG
Notes AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.
Alias Names AIF Antibody: CNK, KSR, CNK1, Connector enhancer of kinase suppressor of ras 1, CNK homolog protein 1, Connector enhancer of KSR 1
Product Type Antibodies Primary
Protocol AIF antibody can be used for detection of AIF by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Purification AIF Antibody is affinity chromatography purified via peptide column.
References Zamzami N and Kroemer G. Condensed matter in cell death. Nature 1999; 401:127-8.
Research Area Cell Death
Size 0.02 mg
Source / Host rabbit
Species Reactivity human, mouse, rat
Storage AIF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Substrate / Buffer AIF Antibody is supplied in PBS containing 0.02% sodium azide.
Product Page Updated 2019-01-08T13:53:07.487Z

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