Article No
155-1703-07
Application | WB |
Article No | 155-1703-07 |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
Concentration | 2 mg/ml |
Description | Lung Tissue Lysate (Tumor) |
Supplier | ProSci |
Format | Liquid |
Notes | Lung tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Previous Article No | 155-1703-07-0.1 |
Product Type | Lysates |
Sample Type | Tissue |
Shipping Information | BLUE ICE |
Size | 0.1 mg |
Storage | 4°C, -80°C |
Substrate / Buffer | Lung Tissue Lysate (Tumor)is supplied in SDS sample buffer containing 5% β-mercaptoethanol. |
Tissue Type | Lung |
Product Page Updated | 2024-01-03T13:34:25.701Z |