Article No
155-1701-05
Lung Tissue Lysate (Normal)
Lung Tissue Lysate (Normal)
Lysates
Specifications
| Application | WB |
| Article No | 155-1701-05 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Concentration | 2 mg/ml |
| Description | Lung Tissue Lysate (Normal) |
| Supplier | ProSci |
| Format | Liquid |
| Notes | Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
| Previous Article No | 155-1701-05-0.1 |
| Product Type | Lysates |
| Sample Type | Tissue |
| Shipping Information | BLUE ICE |
| Size | 0.1 mg |
| Storage | 4°C, -80°C |
| Substrate / Buffer | Lung Tissue Lysate (Normal)is supplied in SDS sample buffer containing 5% β-mercaptoethanol. |
| Tissue Type | Lung |
| Product Page Updated | 2024-01-03T13:34:25.701Z |
