NIH/3T3 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Procedures for immunohistochemistry can be directly applied.
Source / Host
NIH/3T3 cell slide can be stored for up to 6 months at 4˚C under desiccate conditions.
Mouse NIH/3T3 cells were cultured in DMEM with 4 mM L-glutamine and harvested at the log phase of growth. The NIH/3T3 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each well’s surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 NIH/3T3 cells. A follow-up heat dehydration process was done to ensure the attachment of NIH/3T3 cells and the stability of cellular proteins.
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The delivery time for this item is approximately 5-8 business days. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.