Article No
155-1403-0
Specifications
| Application | WB |
| Article No | 155-1403-0 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Concentration | 2 mg/ml |
| Description | Brain Lysate (0 Days Old) |
| Supplier | ProSci |
| Format | Liquid |
| Notes | Brain tissue lysate (0 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
| Previous Article No | 155-1403-0-0.1 |
| Product Type | Lysates |
| Sample Type | Tissue |
| Shipping Information | BLUE ICE |
| Size | 0.1 mg |
| Storage | 4°C, -80°C |
| Substrate / Buffer | Brain lysate (0 Days Old) is supplied in SDS sample buffer containing 50 mM DTT. |
| Tissue Type | Brain |
| Product Page Updated | 2024-01-03T13:34:25.701Z |
