Article No
155-1282
Specifications
| Application | WB |
| Article No | 155-1282 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Concentration | 2 mg/ml |
| Description | 3T3 (NIH) Lysate |
| Supplier | ProSci |
| Format | Liquid |
| Notes | 3T3 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The 3T3 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
| Previous Article No | 155-1282-0.1 |
| Product Type | Lysates |
| Shipping Information | BLUE ICE |
| Size | 0.1 mg |
| Storage | Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
| Substrate / Buffer | 3T3 (NIH) lysate is supplied in SDS sample buffer containing 50 mM DTT. |
| Tissue Type | NIH/3T3 (embryo) |
| Product Page Updated | 2023-01-10T14:15:05.841Z |
