Article No
15-1019-0.1
CpG ODN (1668), TLR9 ligand (Class B)
CpG ODN (1668), TLR9 ligand (Class B)
Chemicals & Biochemicals
Specifications
| Application | FA |
| Article No | 15-1019-0.1 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | CpG ODN (1668), TLR9 ligand (Class B) |
| Supplier | Abeomics |
| Notes | Sequence: 5'-tccatgaCGttcctgatgct-3' (Class B ODN 1668: Bases are phosphorothioate.) Synthetic oligodeoxynucleotides (ODN) containing unmethylated deoxycytosine-deoxyguanosine (CpG) motif are equivalent to bacterial DNA in the immunostimulatory activity, which can induce innate immunity via Toll-like receptor 9 (TLR9) in mammals. There are three major classes of CpG ODNs, and each ODN class exhibits different stimulatory effects on immune cell activation. Class A ODNs are potent inducers of IFN-alpha that leads to the plasmacytoid dendritc cell (pDC) maturation. Class B ODNs are relatively weak inducers of type I IFNs but strong stimulators of human B cells and monocyte maturation. Class C ODNs combine elements of both Classes A and B ODNs, which can induce IFN-alpha in pDC and activation of B cells. |
| Product Type | Chemicals & Biochemicals |
| Protocol | A. CpG ODN 1668-mediated mouse TLR9 activation in NF-kB Leeporter™- RAW 264.7 cells (Figure 2). 1. Harvest NF-kB Leeporter™- RAW 264.7 cells and seed cells into a white solid-bottom 96-well microplate in 100 µl of growth medium at 5 x 104 cells/well. 2. Incubate cells at 37°C in a CO2 incubator for overnight. 3. The next day, stimulate cells with various amounts of CpG ODN 1668. 4. Incubate at 37°C in a CO2 incubator for 6-16 hours. 5. Add 30-50 µl of luciferase assay reagent per well. 6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer. |
| Size | 0.1 mg |
| Species Reactivity | mouse, rat |
| Storage | -20°C |
| Product Page Updated | 2024-02-06T07:35:53.072Z |
