Article No
111-476-144
DyLight 405-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot)
DyLight 405-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot)
Antibodies Secondary
Specifications
| Application | IF, FC |
| Article No | 111-476-144 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone Type | polyclonal |
| Conjugation | DyLight 405 |
| Description | DyLight 405-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot) |
| Recommended Dilution | 1:100 - 1:800 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
| Supplier | Jackson ImmunoResearch |
| Format | Affinity Purified |
| Immunogen | IgG (H+L) |
| Isotype | IgG |
| Notes | F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, mouse and rat serum proteins, but it may cross-react with immunoglobulins from other species. |
| Product Type | Antibodies Secondary |
| Protocol | 1:100 - 1:800 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
| Purity | AffiniPure |
| Shipping Information | RT |
| Size | 0.75 mg |
| Source / Host | goat |
| Species Reactivity | rabbit |
| Storage | 4°C |
| Substrate / Buffer | 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 |
| Product Page Updated | 2023-12-29T14:15:50.354Z |
