Antibodies Primary

14 -3 -3

NEW
Polyclonal Antibody to 14-3-3

Article No

11-1023-100

Application

WB

Species Reactivity

human

Size

100 µg

Source / Host

rabbit

Product insert

Suggested protocols

Specifications

Application WB
Article No 11-1023-100
Clone Type polyclonal
Description Polyclonal Antibody to 14-3-3
Supplier Abeomics
Format Purified
Immunogen A partial length recombinant 14-3-3 protein (amino acids 20-225) was used as the immunogen for this antibody.
Isotype IgG
Notes YWHAQ, also known as 14-3-3 tau (14-3-3tau) is a a phosphoserine-binding protein and a member of the 14-3-3 family of proteins. The 14-3-3 family members are dimeric phosphoserine/ phosphothreonine-binding proteins, which are involved in a very diverse spectrum of signaling pathways. YWHAQ plays a critical role in the regulation of E2F1 stability. It binds to Ser31-phosphorylated E2F1 and inhibits the ubiquitination of E2F1 during DNA damage. The binding of YWHAQ to E2F1 interferes with the function of an E31 ligase and therefore inhibits the ubiquitination and degradation of E2F1. This interaction is required for the expression and induction of several E2F1 apoptotic target genes as well as apoptosis during DNA damage.
Alias Names YWHAQ
Product Type Antibodies Primary
Protocol Western blot analysis: 2-4 µg/ml
Purification Protein A Chromatography
Research Area Cell Death
Size 100 µg
Source / Host rabbit
Species Reactivity human
Storage Store the antibody at 4⁰C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freezing and thawing
Substrate / Buffer 100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
UniProt Number P27348
Product Page Updated 2019-04-24T15:22:26.078Z

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