Article No
101-035-165
Peroxidase-conjugated AffiniPure Goat Anti-Bovine IgG (H+L) (min X Ar Hms,Hu,Ms,Rat Sr Prot)
Peroxidase-conjugated AffiniPure Goat Anti-Bovine IgG (H+L) (min X Ar Hms,Hu,Ms,Rat Sr Prot)
Antibodies Secondary
Specifications
| Application | ELISA, ICC, IHC, WB |
| Article No | 101-035-165 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone Type | polyclonal |
| Conjugation | HRP |
| Description | Peroxidase-conjugated AffiniPure Goat Anti-Bovine IgG (H+L) (min X Ar Hms,Hu,Ms,Rat Sr Prot) |
| Recommended Dilution | ELISA 1:5,000 - 1:100,000, ICC 1:500 - 1:5,000, IHC 1:500 - 1:5,000 |
| Supplier | Jackson ImmunoResearch |
| Format | Affinity Purified |
| Immunogen | IgG (H+L) |
| Isotype | IgG |
| Notes | Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule bovine IgG. It also reacts with the light chains of other bovine immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with armenian hamster, human, mouse and rat serum proteins, but it may cross-react with immunoglobulins from other species. |
| Product Type | Antibodies Secondary |
| Protocol | 1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substrates Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
| Purity | AffiniPure |
| Shipping Information | RT |
| Size | 1.5 ml |
| Source / Host | goat |
| Species Reactivity | cow/bovine |
| Storage | 4°C |
| Substrate / Buffer | 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 |
| Product Page Updated | 2023-12-29T14:15:50.354Z |
