Alexa Fluor® 647-conjugated conjugated Streptavidin

Alexa Fluor® 647-conjugated conjugated Streptavidin

Secondary Reagents

Article No

016-600-084

Size

1 mg

Shipping Information

RT

Article No

016-600-084

Size

1 mg

Shipping Information

RT

Specifications

Article No 016-600-084
Country Availability SE, FI, DK, NO, IS, EE, LV, LT, RU
Conjugation Alexa Fluor 647
Description Alexa Fluor® 647-conjugated conjugated Streptavidin
Recommended Dilution Western Blot:- 0.5-2 µg / ml Histo-/Cyto-Chemistry:- 0.5-2 µg / ml Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Supplier Jackson ImmunoResearch
Notes Steptavidin, a bacterial protein isolated from Streptomyces avidinii, is similar to egg-white avidin in its ability to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties. Conjugates of streptavidin are recommended for use with Biotin-SP-conjugated antibodies and Biotin-SP-conjugated ChromPure proteins.
Product Type Secondary Reagents
Protocol Western Blot:- 0.5-2 µg / ml Histo-/Cyto-Chemistry:- 0.5-2 µg / ml Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Shipping Information RT
Size 1 mg
Storage Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Substrate / Buffer 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Product Page Updated 2022-04-27T07:10:34.190Z
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