World’s First 2′,3′-Cyclic GAMP EIA Kit

news July 29 2019

 

The innate immune system is the body’s first defense against viral infections and it is dependent upon a group of pattern recognition receptors. Cyclic GMP-AMP Synthase (cGAS) responds to the presence of viral DNA in the cytosol by producing 2’,3’cGAMP. Cyclic GAMP in turn binds to STING (STimulator of Interferon Genes), leading to TBK1-mediated IRF3 activation and the production of type 1 Interferon. Understanding the STING pathway and the role cGAMP plays in the innate response to viral infection is of great interest as a possible mechanism for pharmaceutical intervention to treat cancer, inflammatory and autoimmune diseases, and infections.

Arbor Assays introduced the world’s first EIA kit to measure 2’,3’-cyclic GAMP for analysis of STING signaling. The cGAS-cGAMP-STING pathway plays a critical role in pathogen detection and in physiological conditions such as metabolic dysregulation, autoimmunity, and cancer. This kit joins a series of cell signaling assay kits used to directly quantitate intracellular signaling molecules including cyclic AMP, cyclic GMP, Nitric Oxide, PGE2, as well as an important signal cascade enzyme.

Features

  • Use: Measure cGAMP in STING signaling
  • Range: 50 pmol/mL – 0.205 pmol/mL
  • Time to Answer: 2.5 Hours
  • Sensitivity: 0.08 pmol/mL, < 5 fmol/well
  • Samples/Kit: 39 or 231 in Duplicate
  • Stability: Liquid, 4°C Stable Reagents

Standard curve